Polarized light-emitting films have potential applications in liquid crystal displays and can significantly reduce energy consumption. The key to the preparation of polarized light-emitting films lies in the ordered orientation of the light-emitting materials. In this paper, the anisotropic CsPbBr₃ nanowires were used as the luminescent material, and the off-center spin-coating method was used to perpare the polarized luminescent film. The fluorescence spectrometer was used to test the degree of polarization of the film, and scanning electron microscope was used to observe the orientation of the nanowires. The orientation of the nanowire film was optimized by changing the substrates, nanowire dispersion concentration, and solvent composition, and a good fluorescence polarization degree was obtained.